Fluorescent

Phosphor

Phosphor

And for that reason, we cover the LED with a phosphor cap.

Luminous poisoning? Go on, do what I tell you.

That's a third of the U.S. population.

The GlowintheDark Flashlight!

Yes. (Amemiya Hotaru) Good work. (Sachiko) Good work.

You must steel yourself for a shock, Mr. Bigelow. Well go on, Doctor, what is it you're trying to tell me?

And now, boom, the tumor labels itself and it gets fluorescent.

And this is what I think is the magic of fluorescence.

What's the trouble here?

We're trying to preserve nerves we can't see where they are.

And so what we did is, we take tissue culture cells and infect them with various viruses, and we take the stuff and fluorescently label the nucleic acid, the genetic material that comes out of these tissue culture cells mostly viral stuff and stick it on the array to see where it sticks.

Harry's really slow to catch on.

Turn it on turn it off.

In 2008, the Nobel Prize in Chemistry was awarded for work done on a molecule called green fluorescent protein that was isolated from the bioluminescent chemistry of a jellyfish, and it's been equated to the invention of the microscope, in terms of the impact that it has had on cell biology and genetic engineering.

But the pay off is really enormous.

The reason that Vibrio fischeri is doing that comes from the biology.

Tell them to prepare a stomach wash for luminous poisoning.

But even before I peel that muscle away, you saw that there was a tumor underneath.

This bacterium has the special property that it makes light, so it makes bioluminescence,

Thermosalinographs, fluorometer, carbon analyzer...

Since August 2012, harmful phosphors have been found in the instant noodle package cups from every famous brand sold in China's supermarkets.

This is cultures, identical cultures, of cervical cancer cells.

Now bright, now dimmed, was the moonlight pale,

I must replace that fluorescent lamp.

Well, the GlowintheDark Flashlight, that was mine.

And this little piece was shown just for the spring session.

Come.

Well we took the manuscript, and we decided to image it in X ray fluorescence imaging.

A firefly?

Hotaru?

It's not that long ago that our lighting was just done with these kinds of lamps.

And now, one way goes from orange to yellow, but the other way goes from orange to...neon yellow.

This fluorescent lamp is starting to flicker. We'll have to replace it.

Now, I've filled out almost all of this deterministic equivalent.

But one of the most popular ones involves green fluorescent protein.

We take that tissue, we purify the RNA out of it using some basic technology, and then we put a florescent tag on it.

Hotarubi!

Hotarubi!

Hotarubi...

It used to be a weight room, so there were rubber floors below, acoustic tile ceilings and fluorescent lights.

And I came in half an hour beforehand so I could have it put on and kind of get used to it, and they got it bent so it's not hitting my chin.

Compare the night sky.